Supporting data for "Bioenergetic study of Wolffia photosynthesis"

This thesis studied Wolffia photosynthesis through the lens of NADP(H) and stromal ATP dynamics. A biolistic transformation protocol was optimized to transform promoter:GUS plasmids and plasmids containing p35S driven AT1.03 (a FRET-based ATP sensor) into Wolffia. Promoter:GUS assay was conducted to assess whether the promoter of a nucleotide transporter protein (NTT) was active in mature/mother fronds of Wolffia. Stromal and cytosolic ATP were measured via confocal microscopy of AT1.03, yielding FRET ratios as proxy measurements of ATP levels. NADP(H) levels and chlorophyll content in Wolffia were assayed in parallel with the model plant, <i>A. thaliana,</i> as a point of comparison.This dataset includes:photos of biolistically transformed <i>Wolffia australiana</i> (p35S:GUS and pNTT:GUS) after GUS histochemical staining in TIF format taken by Olympus SZX16 stereomicroscope equipped with Nikon FI3 camera.photos of Wolffia in CZI format taken via confocal microscopy (Carl Zeiss LSM 980) which include Wolffia expressing AT1.03 after inhibitor treatments (oligomycin, DCMU+oligomycin, or DBMIB+oligomycin), or no treatment (control).tabulated data in XLSX format including GUS signal counts, transformed Wolffia frond/total fronds counts, AT1.03 FRET ratios as measured by Redox Ratio Analysis software (Mark Fricker), electron transport rate as measured by Imaging-PAM MAXI version (Walz), NADP(H) assay results, and chlorophyl assay results.<br>