Cytotoxic and genotoxic potential of Avobenzone and Octocrylene on human skin fibroblast cells raw data file

This dataset presents the raw data used to prepare charts for the following paper: Evaluation of cytotoxic and genotoxic potential of Avobenzone and Octocrylene on human skin fibroblast cells. In this study, we investigate the cytotoxic and genotoxic effects of the organic UVA filter avobenzone (AVB), and the UVB filter octocrylene (OCT), found alone or in combination in different sunscreen formulations of CCD-1118Sk: human healthy skin fibroblast cells. The data shows the cytotoxic and genetic potential of Avobenzone and Octocrylene alone and in combination. Absorbance was measured using a microplate reader at a wavelength of 450 nm, and cell proliferation curve was drawn. For the XTT assay, after blank subtraction, percent growth inhibition of cells was calculated as follows: Growth inhibition (%)=( 1-Aexp group)/(A control)× 100 . The results of the Comet test were calculated by averaging the data obtained for each dose of each parameter. The intracellular ROS assay results were also calculated by averaging the data obtained for each dose. The graphs are available in the article.本数据集提供了为以下论文制作图表所用的原始数据：《阿伏苯宗（Avobenzone）与奥克立林（Octocrylene）对人皮肤成纤维细胞的细胞毒性与遗传毒性潜力评估》。本研究以人源健康皮肤成纤维细胞CCD-1118Sk为模型，探究了单独或复配于不同防晒制剂中的有机UVA过滤剂阿伏苯宗（avobenzone, AVB）与UVB过滤剂奥克立林（octocrylene, OCT）所产生的细胞毒性与遗传毒性效应。本数据集涵盖了阿伏苯宗与奥克立林单独及复配使用时的细胞毒性与遗传毒性相关数据。实验采用酶标仪在450 nm波长下检测吸光度，并绘制细胞增殖曲线。针对XTT检测法（XTT assay），在扣除空白吸光度后，细胞生长抑制率按下式计算：生长抑制率(%) = (1 - A实验组 / A对照组) × 100。彗星试验（Comet test）的结果通过对各参数下各剂量组获取的实验数据取平均值进行计算得到。细胞内活性氧（reactive oxygen species, ROS）检测实验的结果同样通过对各剂量组获取的实验数据取平均值进行计算得到。相关图表已刊载于该研究论文中。