Metagenomical strain-level analysis of the laboratorial mouse gut microbiota. Metagenomic dataset of the laboratorial mouse gut microbiota

The laboratorial mouse harbors a unique gut microbiota with potential value for human microbiota-associated studies. In this study, we collected faeces and gut content samples from the laboratorial C57/BL mice and generated a total of 33.9 Gbp high-quality data using whole-metagenome shotgun sequencing. Using the methodology of metagenome-assembled genomes (MAGs), we reconstructed 55 nonredundant MAGs from the sequenced samples, including 46 MAGs belonging to uncultured species. These MAGs included members across all phyla in mouse gut (i.e. 23 Firmicutes, 22 Bacteroidetes, 7 Proteobacteria, 1 Deferribacteres, 1 Verrucomicrobia, and 1 Tenericutes), allowing a strain-level delineating of the mouse gut microbiota. The MAGs revealed distinctive genomic and functional characteristics of mouse’s Bacteroidetes and Firmicutes strains compared to human’s. Moreover, we also showed the genomic characteristics of several representative strains belonging to the rare phyla in mouse gut microbiota, including strainsof Mucispirillum schaedleri, Parasutterella excrementihominis, Helicobacter typhlonius, and Akkermansia muciniphila. Our findings highlighted a unique ecosystem in the gut of laboratorial mice that obviously differed with the human gut flora at the strain level. These outcomes may be benefitial to researches based on laboratorial mouse models.