Analysis of an HCA-7 derived cell line (CC-CR) and cell lines generated with knockout of miR-100, miR-125b, or both miRNAs. (smallRNAseq_human_miR_KO.8556)

We report the results of RNA-Seq from an HCA-7 derived cell line with high levels of miR-100 and miR-125b (CC-CR) and knockout cell lines generated from the high expressing cells. CC-CR cells had miR-100, miR-125b or both miRNAs knocked out using CRISPR Cas9. Since miRNAs function to negatively regulate their target, the knockout cell lines should have the potential mRNA targets upregulated in comparison to CC-CR cells. Results were used to identify potential mRNAs that were upregulated in the knockout cell lines compared to CC-CR cells Overall design: miRNAs of interest were knocked out using CRISPR-Cas9. RNAseq was conducted to determine potential targets of the miRNAs