Effect of maternal treatment on gene expression differences in Bacillus thuringiensis infected pooled larvae

This study examines gene expression differences between infected larvae from different maternal infection backgrounds to try to understand the immunological basis of invertebrate immune priming. Female Tribolium castaneum beetles were either jabbed with sterile saline, heat killed Bt, or left naïve. 15 Offspring from the first and second broods were infected with Bt and 24 hours later pooled by treatment and flash frozen Beetles from saline and primed maternal backgrounds are compared to naïve infected Pooled whole larvae, custom array for T. castaneum, 1 replicate per treatment comparison. Same naïve sample used for both arrays