Identification of GFP-SPT-2 binding sites in the Caenorhabditis elegans genome

We aimed at investigating whether the newly identified C. elegans SPT2 histone chaperone controls chromatin structure. We decided to profile SPT-2 binding sites to identify its genomic targets and we performed GFP ChIP-seq on a C. elegans strain expressing endogenously GFP-tagged SPT-2, using WT N2 untagged worms as control for binding specificity. We performed GFP ChIP-seq on synchronised adult worms expressing endogenously GFP-tagged SPT-2, or WT N2 untagged controls. Two independent replicates of the experiment were performed.