Analysis of the protein sequences and structures of Rho GTPases.

(A) Schematic representation of atypical Rho GTPases. Boxes in color are characteristic structures: the Rho GTPase domain (blue), CAAX box (orange), BTB domains (green), EFH domains (pink) and second GTPase domain in Miro (light blue). The first GTPase domain resembles Rho GTPases, and the second is more related to the Rab family of small GTPases [21]. (B) Sequence alignment of the Rho family. The amino acid sequences of Rho GTPases were aligned using ClustalW. The highlighted (cyan) residues are important for GTPase activity. The characteristic structures are marked. The amino acid sequences of RhoBTB and Miro proteins are truncated at the C-terminus. Red shaded and red letters indicate identical and similar amino acids, respectively.