Non-programmed dissemination of cancer cell clusters induce malignant properties through Integrin beta 1 activation

We found that epithelial cluster recruiment soluble fibronectin and then activating beta1 Integrin induce epithelial-mesenchymal transition and enhance cancer cells’ malignant after cell dissemination. Non-programmed dissemination of cluster cells triggers ERK cascade and then accelerate tumor growth and metastasis. Our finding reveals that cell cluster plays a vital role in the non-programmed dissemination of breast cancer cells’ metastasis, offering a potential new therapeutic target for metastasis patients. MCF7 Breast Cancer cells (ATT) collected as single cells (SC) or cluster cells (CL) and then growth on suspension (DE-SC and DE-CL), then reattached on regular plate to mimic disseminated tumor cells ( RE-SC and RE-CL). Total 6 cell samples were generated and did RNA sequencing