Comparison of gene expression profile of human blastocyst between young and aging infertility patients

The pregnancy rate of women decreases with age due to declining quality of oocytes and embryos. However, there is no established method to improve pregnancy rate in aging women. Here, we performed microarray analysis for detecting candidate genes affected the quality of aging embryos. Based on microarray analysis using young and aging human embryos at the same morphological grade, 820 genes showed > 5-fold change in aging human blastocysts. Among these genes, C-X-C motif chemokine 5 (CXCL5), one of SASP factors, showed 7.2-fold increases in aging human blastocysts. These findings indicate CXCL5, one of senescence-associated secretory phenotype (SASP) factors, partially responsible for the decline in embryo implantation potentia. Gene expression profile of human blastocysts were compared. Blastocysts were obtained from infertility patients which were separated in two age group (young group; 25-37 years of age and aging group; 38-45 years of age). Five blastocysts were used for samples in each age group.