Figure_2.jpg

Bmal1 knockout increases p65 expression in periodontitis. (a) Immunofluorescence staining of p65 in the maxilla. Green fluorescence shows the expression of p65. (b) Statistical analysis of the integrated density/area of p65-positive cells in (a). (c) Statistical analysis of the percentage of p65-positive cells in (a). (d-f) ELISA was used to detect the protein expression levels of TNFα, IL1β and IL6. (g) qRT-PCR was used to detect the mRNA expression levels of p65. PDL: periodontal ligament; DAPI: nuclear dye, blue. White arrows show p65-positive cells. *p < 0.05, ***p < 0.001, N.S.: No statistically significant difference. ELISA: enzyme-linked immunosorbent assay. qRT-PCR: Quantitative reverse-transcription polymerase chain reaction.