Effect of gut microbiota colonizing on gene expression in the porcine hypothalamus

To investigate the impact of gut microbiota deleting and colonizing on hypothalamic health and function, the tissue samples from the germ-free (GF) pigs and the GF pigs colonized gut microbiota (CG) are used to perform whole RNA-seq for gene expression analysis We then performed long RNAs and small RNAs expression profiling analysis using data obtained from the RNA-seq of 3 different hypothalamus samples from the two groups of pigs Comparative gene expression profiling analysis of RNA-seq data in the hypothalamus for the germ free (GF) pigs and the pigs with gut microbiota