Transcription profiling of mouse atrioventricular junction gene expression at embryonic day 10.5-11.0

Goals of this study were to identify new candidates involved in the development of the Atrioventricular cushions in the mouse heart. Experiment Overall Design: The atrioventricular junction and ventricular region were dissected from 80 embryonic day 10.5-11.0, wild type, C57BL/6 mouse hearts. RNA was isolated from the respective regions, amplified using the Arcturus RiboAmp HS RNA amplification kit, and hybridized to Affymetrix MOE430A expression chips in duplicate. Comparison between atrioventricular gene expression and ventricular gene expression will allow for the identification of genes necessary in the development of the AV cushions.本研究旨在鉴定参与小鼠心脏房室垫（Atrioventricular cushions）发育的新型候选因子。 实验整体设计：从80只胎龄10.5~11.0天的野生型C57BL/6小鼠心脏中，分离得到房室交界区与心室区域。从上述区域提取RNA，采用Arcturus RiboAmp HS RNA扩增试剂盒进行扩增，随后以双重复形式与Affymetrix MOE430A表达芯片进行杂交。通过对比房室区与心室区的基因表达谱，可鉴定出房室垫发育过程中所必需的基因。