Single cell RNA Sequencing of murine tdTomato positive skin cells with or without HIRA knockout [dataset 1]
收藏Alliance of Genome Resources2026-08-01 收录
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Skin samples from wild type or HIRA knockout mouse embryos were dissociated to give cellular suspensions, then single cell RNA sequencing was performed using the 10X platform. Method: Cellular suspensions of skin cells from wild type and HIRA knockout mice were prepared, then sequencing was performed using the Chromium 10X system. Data were then processed using Cellranger version 2.2.0. Isolated single cells from E15.5 skin from wild type and HIRA knockout mice were sequenced using the 10X Genomics platform.
本数据集以野生型或HIRA基因敲除(HIRA knockout)小鼠胚胎的皮肤样本为材料,经解离制备为细胞悬液后,采用10X平台开展单细胞RNA测序。实验方法:制备野生型与HIRA基因敲除小鼠的皮肤细胞悬液,通过Chromium 10X系统完成测序,测序数据使用Cellranger 2.2.0版本进行处理;此外,针对野生型及HIRA基因敲除小鼠胚胎发育第15.5天(E15.5)的皮肤分离得到的单细胞,采用10X Genomics平台完成测序。



