Circadian regulation of gene expression in the SCN (RNA-seq)

We performed a circadian RNA expression profile of the mammalian biological clock, the suprachiasmatic nucleus (SCN) in C57/BL6 mice, at 2-hour resolution using microarrays, and at 6-hour resolution using RNA-seq. 8 samples total covering 8 time points, with no replicates. SCN samples from mouse brains collected every 6 hours for 2 days (8 samples total).本研究针对C57/BL6小鼠体内的哺乳动物生物钟核心结构——视交叉上核（suprachiasmatic nucleus, SCN），开展节律性RNA表达谱分析：采用基因芯片（microarrays）获得2小时间隔分辨率的表达数据，同时通过RNA测序（RNA-seq）获取6小时间隔分辨率的表达数据。本次实验共设置8个时间点，采集8个样本且无生物学重复；前述样本均为连续2天每6小时从小鼠大脑中分离得到的视交叉上核组织。