Cashew gum nanoparticles effects on cooled stallion semen.

The stallion sperm is widely susceptible to cold shock during storage at low temperatures. Thus, this study added CG or nanoparticles (NP) containing CG to the extender before cooling due to verified if it was possible to improve sperm quality in stallion semen. We worked with 6 treatment groups (control, a-tocopherol TOC, CG1, CG0.5, NP1 and NP0.5), stored in cryotubes at 4°C, and analyzed on the day, after 24h and 48h. The datas show the results obtained by CASA (computer-assisted sperm analysis) and Flow cytometry assays.种公马精子在低温储存过程中极易遭受冷休克损伤。鉴于此，本研究在降温前向精液稀释液中添加CG或含CG的纳米颗粒（nanoparticles，NP），以验证其是否可改善种公马精液的精子质量。本研究共设置6个处理组：对照组、α-生育酚（TOC）组、CG1组、CG0.5组、NP1组及NP0.5组，所有样本均储存于4℃冻存管中，并分别于处理当日、24小时及48小时后开展分析检测。本研究所得数据涵盖了计算机辅助精子分析（computer-assisted sperm analysis，CASA）与流式细胞术检测的实验结果。