A streamlined approach to antibody novel germline allele prediction and validation. Homo sapiens

High quality antibody repertoire sequencing analysis requires not only accurate sequencing data, but also the correct set of reference germline allele sequences to align to to assign mutations. The currently available public database of germline allele, while expansive and useful, is not comprehensive. Here, we developed a simple yet effect method for predicting novel germline allele sequences from antibody repertoire sequencing data and validating them through amplification and sequencing of non-rearranged genomic DNA.