Expression Profiling of MAP Kinase-Mediated Meiotic Progression in C. elegans

A unique allele of MAP kinase, mpk-1(ga111), displays a reversible, temperature-sensitive, tissue-specific defect in progression through meiotic prophase I. We performed gene expression profiling on mpk-1(ga111) animals at multiple times after shift to permissive temperature to identify candidate downstream effectors of MPK-1 signaling in the germline. This analysis delineated a cohort of genes whose expression requires MPK-1 (MAP kinase) signaling in germ cells in the pachytene stage of meiosis I. Keywords: Timecourse of C. elegans mpk-1 mutants丝裂原活化蛋白激酶（MAP kinase）的独特等位基因mpk-1(ga111)，在减数分裂I前期进程中表现出可逆、温度敏感且组织特异性的缺陷。我们将携带mpk-1(ga111)等位基因的样本转移至许可温度后，于多个时间点开展基因表达谱分析，以鉴定生殖系中MPK-1信号通路的候选下游效应因子。该分析确定了一组基因，这些基因的表达在减数分裂I期粗线期的生殖细胞中依赖于MPK-1（丝裂原活化蛋白激酶）信号通路。关键词：秀丽隐杆线虫（C. elegans）mpk-1突变体的时程分析