Locomotor behavior summary for <i>tim-Gal4</i> mediated RNAi knockdown of PDF.

Loss of PDF from the l- and s-vLNs by tim-Gal4-driven expression of uas-Dcr2 and uas-PDFrnai was associated with the major locomotor rhythm effects of Pdf01 under DD and LD 12∶12 conditions. y w,uas-Dcr2/y;tim-Gal4/+;uas-PDFrnai/+ flies displayed reduced rhythmicity under DD relative to all three control lines. ANOVA revealed a significant difference in τ between these four genotypes (p<0.0001) and a Tukey's test revealed that y w,uas-Dcr2/y;tim-Gal4/+;uas-PDFrnai/+ flies had a significantly shorter free-running period than all other genotypes (p<0.001 in for all comparisons). ANOVA also revealed a significant difference between genotypes in evening peak phase in LD 12∶12 (p<0.0001) with y w,uas-Dcr2/y;tim-Gal4/+;uas-PDFrnai/+ flies displaying a significantly early peak relative to all other genotypes (Tukey's test, p<0.001 for all comparisons).