Transcriptomic changes of optic nerves under crush during hibernation stage. Transcriptomic changes of optic nerves under crush during hibernation stage

we compared the gene expression changes of crushed optic nerves from hibernating and awake squirrels. Overall design: Optic nerves from hibernating and awake 13-lined squirrels were crushed, and total RNAs were extracted and sequenced 3 days after treated. Three replicates were included for hibernation group and awake group. Paired-end library following Illumina TruSeq stranded protocol was constructed, and subjected to RNA-Seq.