16S RNA amplicon sequencing of mucosal, stool and saliva samples from patients with IBD and non-IBD pathology

In 120 patients with IBD, 16S rRNA gene amplicon sequencing was performed on samples from stool, intestinal mucosa and saliva. Patients with non-IBD inflammation of the bowel (N=28) and healthy subjects (N=67) served as controls. A total of 480 16S profiles were analyzed. The results were evaluated with multiple clinical and pathological parameters and potential confounders were considered. The aim of the study was to identify differences and similarities in the individual compartments and to establish clinical associations.