Gossypium hirsutum Raw sequence reads

This study employs transcriptome sequencing to elucidate the molecular mechanism by which the GhBsr-d1L7 gene confers resistance to Verticillium wilt in upland cotton (Gossypium hirsutum). The experimental design involved virus-induced gene silencing (VIGS) to knock down the expression of GhBsr-d1L7 in cotton roots.Cotton seedlings were first injected with either TRV2::GhBsr-d1L7 (for gene silencing, the treatment group) or TRV2::00 (the empty vector control group). Fourteen days post-VIGS infiltration, all plants were challenged with the fungal pathogen Verticillium dahliae. Root tissue samples were collected following this inoculation.The dataset includes RNA-seq data from both the GhBsr-d1L7-silenced treatment group and the empty vector control group, with three biological replicates per condition. By comparing the transcriptomic profiles of these two groups, this research aims to identify the key genes and signaling pathways that are regulated by GhBsr-d1L7 during the cotton plant's defense response. The findings are expected to provide crucial insights into the molecular basis of cotton resistance to Verticillium wilt.