Supplementary Table 2 Differentially expressed lncRNAs identified by RNA-seq

Aim: To explore the
expression profiles of lncRNAs and identify novel lncRNAs as biomarkers for
early diagnosis and therapy of hepatocellular carcinoma (HCC). Materials
& methods: Expression profiles of lncRNAs and mRNAs in five paired HCC
and adjacent normal tissues were obtained by RNA-seq. Eight lncRNAs,
including two novel liver-specific lncRNAs (NONHSAT059247.2 and
NONHSAT013897.2), were validated in another 74 pairs of HCC and adjacent
normal tissues by qRT-PCR. Results: The results of qRT-PCR showed that
NONHSAT252133.1, NONHSAT112116.2, and NONHSAT242657.1 were significantly
upregulated in HCC tissues, whereas NONHSAT169790.1, NONHSAT059247.2, and
NONHSAT013897.2 were significantly downregulated. Two liver-specific lncRNAs
demonstrated excellent diagnostic performance: NONHSAT059247.2 (AUC=0.941,
95% CI: 0.902-0.979, P<0.0001), NONHSAT013897.2 (AUC=0.944, 95% CI:
0.906-0.983, P<0.0001).

Conclusion: The liver-specific lncRNAs
NONHSAT059247.2 and NONHSAT013897.2, may provide new biomarkers for the future
study on diagnosis, therapy, and mechanisms of HCC.