Transcriptome Analysis Reveals Potential Protective Effects and Biomarkers of Pien Tze Huang on CCl4-induced Liver Cancer (circRNA)

Pien Tze Huang (PZH) is a traditional Chinese medicine which was widely utilized in Asia for hepatic diseases. We construct two groups liver cancer mice model using CCl4, one group using PZH treatment and another group didn?t treatment. We found that the liver functional indices including ALB, ALT and AST were significantly increased in non-PZH treatment group. HE staining showed that inflammation was emerged surrounding the hepatic vein in the non-PZH treatment group, Masson staining revealed that there are blue collagenous fibre surrounding hepatic vein in the non-PZH treatment group, this result indicated that PZH cured the hepatic inflammation and cancer. We performed the mRNA,LncRNA,ciecRNA and miRNA sequencing aim to explore the drug target using PZH treatment on transcriptome level. 478 protein coding transcripts and 68 lncRNA transcripts were differentially expressed. Our research provided potential transcriptional therapeutic target for the clinical treatment using PZH. Overall design: We constructed two groups liver cancer mice model using CCl4, one group used PZH treatment, another group did not receive treatment. We also did a blank experiment with normal mice using water. In the 22nd week, we selected six mice from each group for RNA sequencing.