Resistance plasmids with pSLT genes identified in this study and properties of the host isolates.

apMVN-STmRV/VR, plasmid Med-Vet-Net Salmonella Typhimurium monophasic resistance-virulence/virulence-resistance. The horizontal transmission capabilities were investigated by conjugation experiments for the following plasmids: RV1, RV2, RV3, RV6, VR1 and VR2; as described in Materials and Methods.bAll resistance genes were demonstrated as plasmid located by Southern blot hybridization on plasmid profiles. Antimicrobial abbreviations: AMP, ampicillin; CHL, chloramphenicol; GEN, gentamicin; STR, streptomycin; SPE, spectinomycin; SUL, sulphonamides; TET, tetracycline and TMP, trimethoprim.cGene cassette organization of unusual sul3-integrons are: In-sul3-type I, dfrA12-orfF-aadA2-cmlA1-aadA1-qacH and In-sul3-type III, estX-psp-aadA2-cmlA1-aadA1-qacH.dOther class 1 integron related sequences tested were intI1 (5′CS), and qacEΔ1, tnpA440, sul1 and sul3 (3′CS).eTransposon and insertion sequence common region gene markers tested by Southern blot hybridization were: tnpR (Tn1721), tnpR-bla (Tn2), merA (Tn21), tetR (Tn10) and rcr2 (ISCR2). Other genes located on the same mobile genetic element were tested by simple and overlapping PCR amplifications as indicated in Table S2, Table S3 and Figure S1.fVirulence genes tested were: spvC, rck, mig5 and srgB characteristic of pSLT virulence plasmid. The spvC gene was mapped on S1- and alkaline lysis-plasmid profiles.gThe sender code and properties already published for each strain is shown in Table S1.hCountries abbreviations (sender Laboratory): UK, the United Kingdom (PHE, Public Health England [formerly HPA, Health Protection Agency] and AHVLA, Animal Health and Veterinary Laboratories Agency); E, Spain (VISAVET-UCM, Centro de Vigilancia Sanitaria Veterinaria-Universidad Complutense de Madrid) and I, Italy (ISS, Istituto Superiore di Sanità).iSource abbreviations: Hu, human; Sw, swine.jBoth isolates have shown identical properties, hence considered as one strain.knd, Inc group not determined. Other small (