Track data for OTI T cell migration in the brain during T. gondii infection

The data contained in this spreadsheet are of the -x, -y, and -z coordinates of fluorescent T cells migrating in the brains of Toxoplasma gondii infected C57BL/6 mice. Mice were infected with a parasite strain expressing ovalbumin (Pru-OVA) for four weeks. Activated GFP-expressing OT-I T cells were transferred to the mice and imaged by multiphoton microscopy seven days later. Microscopy was performed on explanted brain as described in Harris, et al. Nature. 2012. In this dataset, each T cell has a track ID that corresponds to a single cell. The three-dimensional coordinates are provided along with the corresponding time series.本电子表格所载数据为刚地弓形虫（Toxoplasma gondii）感染的C57BL/6小鼠脑内迁移的荧光标记T细胞的-x、-y、-z三维坐标。实验小鼠先接种表达卵清蛋白的弓形虫毒株（Pru-OVA），感染周期为四周；随后向小鼠体内输注活化的绿色荧光蛋白（GFP）标记的OT-I T细胞，并于七天后通过多光子显微镜完成成像。成像操作采用离体脑组织，具体实验流程参照Harris等人2012年发表于《Nature》的研究。本数据集内，每个T细胞均配有唯一对应单个细胞的追踪ID，同时提供三维坐标及其对应的时间序列数据。