ATAC-seq data from DN3 and DN4 cells from WT, HEBcKO, and Rag2-KO mice

Gamma delta T cells that produce IL-17 (gdT17) play essential roles in
barrier immunity and autoimmunity, but the gene networks that install
their functions are not well understood. We have shown that the
transcriptional regulator HEB is required for efficient upregulation of
Id3 in response to TCR signaling in DN3 cells, and that Id3 is essential
for maturation of gdT17 cells. To evaluate how the loss of the HEB affects
chromatin status in response to TCR signaling, we compared thymocyte
subsets from WT and adult HEB fl/fl Vav-iCre (HEB cKO) mice, which lack
HEB in all hematopoietic cells, by ATAC-seq. We sorted DN3 and DN4
thymocytes from adult WT mice and HEB cKO mice. We also sorted DN3 cells
from adult Rag2-/- mice, which cannot rearrange their TCR genes and thus
are unable to develop beyond the DN3 stage.