Targeting metabolic reprogramming by influenza infection for therapeutic intervention Smallwood et. al

Figure 5. BEZ235 mechanism acquired via PI3K/mTOR pathway restriction on metabolism. NHBE cells were infected with CA04 for 17 hours at MOI 1 +/- BEZ235 1hr prior to infection. (A) NHBE were harvested at indicated times and lysates subjected to immunoblotting.图5：经限制代谢的磷脂酰肌醇3-激酶（Phosphatidylinositol 3-kinase, PI3K）/哺乳动物雷帕霉素靶蛋白（Mammalian Target of Rapamycin, mTOR）通路介导的BEZ235作用机制。正常人支气管上皮细胞（Normal Human Bronchial Epithelial cells, NHBE）以感染复数（Multiplicity of Infection, MOI）为1的感染比例，于感染前1小时添加或不添加BEZ235的条件下，感染CA04病毒17小时。(A) 按指定时间收集NHBE细胞，其裂解液用于免疫印迹实验。