Transcription profiling of mouse CBA/CaJ cochlea gene expression profile

This data set is intended as a public resource documenting the identity of roughly 10,000 genes that are abundantly expressed in the mouse cochlea. The data have many uses, including for making comparisons with proteomics studies, and for comparisons of expression profiles with other mouse strains and with other species. The CBA/CaJ strain was chosen because of its lack of known vulnerabilities to premature cochlear degeneration or to extreme reactions to cochlear stresses. It may therefore be considered a "normal" mouse. No experimental manipulations were done on the mice of this study. Contamination of the results by genes expressed in the surrounding petrous bone and from those in blood cells was minimized. Experiment Overall Design: 8 replicates, pooled ears from each of 8 mice本数据集作为公开科研资源，记录了约10000个在小鼠耳蜗中高表达的基因的相关信息。该数据集具备多重应用场景，可用于与蛋白质组学研究开展比对分析，亦能用于与其他小鼠品系、其他物种的基因表达谱进行对比。本研究选用CBA/CaJ品系小鼠，因其未被报道存在早发性耳蜗退变的易感风险，且不会对耳蜗应激产生极端反应，因此该品系可被视作"正常"小鼠模型。本研究未对受试小鼠进行任何实验操作。实验结果受到周围颞骨岩部组织及血细胞中表达基因的污染风险已被降至最低。实验整体设计：设置8组生物学重复，每组样本由8只小鼠的双耳组织混合制备而成。