Peek-A-Boo test: a simple test for assessing the effect of diazepam on medaka (Oryzias latipes) behavior

Abstract: The potential of pharmaceuticals and personal care products to alter the behavior of aquatic organisms is a growing concern. To assess the actual effect of PPCPs on aquatic organisms, the method of a simple but effective behavior test has been required. In this study, we devised a simple behavior test (Peek-A-Boo test) to assess the effect of anxiolytics on model fish behavior (medaka, Oryzias latipes). In the Peek-A-Boo test, we investigated the response of medaka to the picture of a predator fish (donko fish, Odontobutis obscura). The diazepam exposure test (0.8, 4, 20, 100 µg/L) revealed that the time taken for test medaka to approach the picture was 0.22–0.65 fold shortened and the time spent by test medaka in the close area to the picture was 1.8–2.7 fold elongated in all of the diazepam exposure groups compared with the solvent control group (P < 0.05). Hence, it was confirmed that the Peek-A-Boo test could detect the medaka behavioral alteration caused by diazepam with high sensitivity. The Peek-A-Boo test devised in this study is a simple behavior test with high sensitivity for fish behavioral alteration.

Files: coord_images.zip includes the csv files of fish swimming coordinates in the experiment using five other fish images. coord_diazepam.zip includes the csv files of fish swimming coordinates in the diazepam exposure experiment.

摘要：药品及个人护理产品对水生生物行为造成影响的潜在性已成为日益关注的焦点。为了评估个人护理产品（PPCPs）对水生生物的实际影响，迫切需要一种简单而有效的行为测试方法。在本研究中，我们设计了一种简易的行为测试（窥视测试）以评估安神药对模型鱼类行为（斑马鱼，Oryzias latipes）的影响。在窥视测试中，我们探讨了斑马鱼对捕食鱼（鲇鱼，Odontobutis obscura）图像的反应。地西泮暴露测试（0.8、4、20、100 µg/L）显示，与溶剂对照组相比，测试斑马鱼接近图像所需时间缩短了0.22–0.65倍，测试斑马鱼在图像附近区域的停留时间延长了1.8–2.7倍，所有地西泮暴露组均显示出显著差异（P < 0.05）。因此，证实了窥视测试能够以高灵敏度检测地西泮引起的斑马鱼行为改变。本研究中设计的窥视测试是一种针对鱼类行为改变的简易行为测试，具有较高的灵敏度。
文件：coord_images.zip 包含实验中使用的五张其他鱼类图像的鱼游泳坐标的 csv 文件。coord_diazepam.zip 包含地西泮暴露实验中鱼游泳坐标的 csv 文件。