Genome-wide maps of chromatin state in cultured male germline stem cells [scATAC-seq]

We report the application of single-cell ATAC-seq to reconstruct the epigenetic program and identify key transcription factors and cis-regulatory elements associated with the epithelial and mesenchymal heterogeneity and G9a inhibition in cultured male germline stem cells (GSCs). First, we identified epithelial-like and mesenchymal-like clusters in the sample. Pseudotime trajectory also indicated that GSCs could undergo EMT-like process with unique regulators in different stages. Lastly, we showed that G9a inhibition could suppress the EMT-like process in GSCs. Overall design: Chromatin accessibility profiles of untreated GSCs, UNC0638-treated GSCs and GSCs undergoing EMT-like process.