Fast Repetition Rate Fluorometry (FRRF) data collected on the RV L'Astrolabe from 22/10/2008 to 27/10/2008 (Hobart - Dumont d'Urville)

These data were collected on the RV L'Astrolabe (platform code: FHZI) from 22/10/2008 to 27/10/2008 on a trip from Hobart to Dumont D'Urville.Maximum photochemical efficiency of photosystem II (PSII), also called maximum quantum yield of PSII (Fv/Fm), has become one of the most widely utilized fluorescence parameters in phytoplankton research. It represents the potential photochemical efficiency, which is the probability that the light energy captured by the photosynthetic apparatus is being utilized as photochemistry. Fv/Fm has been shown to have an instant response to variations in physical and chemical properties and is interpreted as a diagnostic of the overall health or competence of phytoplankton. Together with the absorption cross section area of PSII and chlorophyll concentration, it can be used to measure primary production (Cheah et al. 2011, Deep Sea Research).Seawater from 3 m depth was supplied continuously from the ship’s clean seawater line. FRR fluorescence yields were measured continuously at 1 minute intervals in dark-adapted state (! 15 minutes dark-adaptation) using a flash sequence consisting of a series of 100 subsaturation flashlets (1.1 μs flash duration and 2.8 μs interflash period) and a series of 20 relaxation flashlets (1.1 μs flash duration and 51.6 μs interflash period).

本数据集采集于2008年10月22日至2008年10月27日，搭载于研究船L'Astrolabe（平台代码：FHZI），航行航线为从霍巴特（Hobart）前往迪蒙·迪尔维尔（Dumont D'Urville）。光系统II（Photosystem II，PSII）的最大光化学效率，又被称为PSII最大量子产额（Fv/Fm），现已成为浮游植物研究中应用最为广泛的荧光参数之一。其表征潜在光化学效率，即光合装置所捕获的光能被用于光化学反应的概率。研究表明，Fv/Fm可对水体物理与化学性质的变化产生即时响应，常被用作评估浮游植物整体健康状态与生存能力的诊断指标。结合PSII吸收截面积与叶绿素浓度数据，该参数可用于水体初级生产力的测定（Cheah等，2011年，《深海研究》（Deep Sea Research））。

实验所用海水持续取自船用洁净海水管路，采集深度为3米。采用快速重复率荧光（Fast Repetition Rate Fluorescence, FRR）技术，对暗适应状态（需经至少15分钟暗适应）下的样本以1分钟为间隔连续测定荧光产额；测定所用闪光序列包含两组：一组为100次亚饱和微闪（单次闪光时长1.1 μs，闪间间隔2.8 μs），另一组为20次弛豫微闪（单次闪光时长1.1 μs，闪间间隔51.6 μs）。