Unraveling human T cell development using scRNA-seq

T cells develop in the thymus from multipotent lymphoid precursors. During this gradual differentiation process thymocytes go through several decision points, before they fully mature into conventional and unconventional T cells. The underlying molecular mechanisms have been investigated in mice, but several details remain unclear in the human context. To be able to study the drivers of thymocyte differentiation in human, we have generated several 3' scRNA-seq libraries from enriched thymocyte populations. Single cell transcriptional libraries were generated from human CD34+, DP, ISP and gdTCR+ thymocytes obtained from a 4 month old donor using the 10X Genomics Chromium 3’ sequencing V3.1 kit.