Regulation of the alveolar regenerative niche by amphiregulin-producing Treg cells (10X)

This study investigates the role of regulatory T cell–derived Areg on lung regeneration following influenza infection. We use single-cell RNA sequencing to capture the heterogeneity of lung mesenchymal populations during influenza infection. Here, we probe the gene expression changes within stromal cells from wildtype mice and in mice that conditionally lack Areg from T cell sources (CD4-cre Areg-flox) at day 8 post infection with PR8/H1N1. n = 1 per genotype. Overall design: Areg-flox and CD4cre Areg-flox mice were infected with H1N1/PR8 influenza and harvested at day 8 post infection. Mesenchymal cells were enriched by negative depletion of CD45+, Epcam+, Ter119+, and CD31+. Enriched cells were delivered to Columbia University Genome Center for 10X bead emulsification.