Parabacteroides distasonis alleviates Clostridioides difficile infection by reshaping bile acid metabolism and the gut microbiota

The aim of this study was to establish a mouse model of Clostridioides difficile infection (CDI) and to investigate the effects of intervention with live Parabacteroides distasonis and its culture supernatant on the fecal bile acid pool using a targeted metabolomics approach. By quantitatively profiling fecal bile acid metabolites, we sought to characterize alterations in bile acid composition and abundance following different interventions in CDI mice, thereby providing metabolomic evidence for the potential role of P. distasonis in modulating host bile acid metabolism.