Data from: A catalogue of proteins released from Asaia bogorensis under two growth conditions

Bacterial secretion systems are essential for growth and responsible for a
plethora of functions, ranging from nutrient acquisition to biofilm
formation, and pathogenesis. Bacteria use Type II secretion systems (T2SS)
to transport folded proteins from the periplasm outside of the cell in a
two-step process. Opportunistic pathogens often secrete virulence factors
through this pathway. In contrast, the Type I secretion system transports
proteins across both the inner and outer membranes in a single step. The
Type 4 secretion system also transports substrates across the entire cell
envelope. In many instances, it also translocates substrates directly into
target cells. Researchers have utilized the Gram-negative bacterium Asaia
bogorensis for paratransgenesis. The bacterium was engineered to secrete
anti-Plasmodium effector peptides to target and destroy the Plasmodium
parasites that cause malaria in the mosquito vector through the T2SS. Here
we identify a catalogue of proteins released by A. bogorensis under two
growth conditions as well as predictions for the secretion systems that
transport them using liquid chromatography with tandem mass spectrometry.
These secretion systems can be utilized in the future to improve
paratransgenesis. We propose utilizing the Type 1 and/or Type 4 secretion
systems for more streamlined effector secretion directly out of the
bacterium. Fusion of anti-parasitic effector peptides to the substrates
encoded at these loci could also serve as an effective minimal
paratransgenic strain not reliant upon plasmids.