MOESM2 of A rapid preparation procedure for laser microdissection-mediated harvest of plant tissues for gene expression analysis

Additional file 2. RNA and qPCR data. RNA quantity, quality and duration of storage in a desiccator at 4 °C prior to LM. Gene expression data including Cq values, calculations and p-values. Gene-specific primer sequences, expected amplicon sizes and qPCR amplification efficiencies calculated based on standard curves of diluted cDNA. Size separation of qPCR amplicons on a 2% agarose gel under constant voltage set to 100 (nucleic acids were stained with GelRed and visualized using the ChemiDoc MP Imaging System (Bio-Rad). The GeneRuler 50 bp Ladder (Life Technologies) was included for size estimation). Melt peaks of qPCR amplicons generated by plotting the negative derivative of the change in fluorescence intensity as a function of temperature.