ZDHHC13 Modulates Tumor Microenvironment Interactions to Suppress Melanoma Metastasis [scRNA-Seq]

This dataset supports a study investigating the role of ZDHHC13 in melanoma development. Using bulk RNA sequencing and 10x Genomics single-cell RNA sequencing, we analyzed how ZDHHC13 influences tumor progression and the tumor microenvironment. These data provide insights into the transcriptomic changes associated with ZDHHC13 activity in melanoma and serve as a resource for understanding gene expression dynamics in both tumor cells and surrounding immune components. Overall design: For single-cell RNA sequencing, ZDHHC13-overexpressing or control B16F10 cells were subcutaneously inoculated into immunocompetent C57BL/6 mice. Tumors were harvested at defined time points, dissociated into single-cell suspensions, and processed using the 10x Genomics platform. This design allowed us to examine both tumor-intrinsic and tumor microenvironment responses to ZDHHC13 expression at single-cell resolution.