Pro-Seq of MEL624 control and PCIF1 KO cells

We analyzed human melanoma cells by PRO-Seq in order to determine the effect of PCIF1 KO in Pol II dynamics Overall design: PRO-seq libraries from 3 independent biological replicates were generated for Control MEL624 cells, PCIF1-KO clone 1 and PCIF1-KO clone 2, using 1 million cells per sample. Samples were spiked with 40,000 Drosophila S2 cells as a normalization spike-in control. Samples were sequenced on the NextSeq using a High Output 75-cycle kit, generating paired end 42 nt reads. Libraries were sequenced to an average read depth of 50 million mappable reads per sample.