Anti-TGFβ antibody improves trabecular architecture in tumor bearing mice tibia and femur.

MicroCT analysis of the tibiae from MDA-MB-231tumor-bearing mice treated for 4 weeks, starting one day after tumor cell inoculation, revealed that suppression of TGFβ by the antibody 1D11 increased trabecular bone volume through increases in trabecular number, and this improved the connectivity of the trabeculae (lack of fenestrations), compared to isotype control. Wilcoxon rank-sum test was used for this analysis. Means and standard deviations by group for the MDA-MB-231 four week data with p-values from Wilcoxon rank-sum tests. Quantitative analysis of microCT data from MDA-MB-231 tumor-bearing mice treated with 13C4 or 1D11 for weeks. Trabecular bone volume (BV/TV), trabecular thickness (Tb.Th*), trabecular number (Tb.N*), and connectivity density (Conn.D), and mean volumetric density of the mineralized tissue (Tb.TMD) were calculated using the Scanco evaluation software.