Analysis of SKMEL-103, AsPC1 and RH-36 cells after treatment with BAY 11-7082

To identify the gene expression changes in NRAS mutant cell line SKMEL-103, KRAS mutant cell line AsPC1 and HRAS mutant cell line RH-36 upon BAY 11-7082 treatment, we analyzed these cell line with either control DMSO or BAY 11-7082 treatment via RNA sequencing. SKMEL-103, AsPC1 and RH-36 cells were plated, samples were either treated with DMSO or BAY 11-7082 (5-micromolar for 48 hours), cells were harvested and were used to prepare the total RNA, followed by RiboRNA depletion with random priming and RNASeq Illumina Hi-Seq 2500 platform, with three biological replicates for each sample.