Human_IPAH_LMM_Arteries

Idiopathic pulmonary arterial hypertension (iPAH) is a fatal disease that is associated with increased vascular resistance due to sustained contraction and enhanced proliferation of pulmonary vascular cells. The current treatment however is still not satisfactory. A better understanding of the involved molecular mechanisms may lead to new therapeutical strategies. Pulmonary arterial walls of healthy human lung tissue and of iPAH lungs were laser-microdissected. The extracted RNA was pooled and subjected to SMART-based RNA preamplification and fluorescence labeling. Gene expressions between iPAH and donors were compared using dual-channel microarrays. Keywords: disease characterization For this study, fresh lung samples from 6 iPAH patients and from 6 healthy donors were available. Laser-microdissected arteries of 3 individuals each were pooled. Dual-color hybridizations were carried out comparing iPAH versus donor including dye-swaps as technical replicates.