five

Data presented in Brinkmann et al., 2021, Aquat. Toxicol.

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These files include all data presented in: Brinkmann et al., 2021. Adsorption of titanium dioxide nanoparticles onto zebrafish eggs affects colonizing microbiota. Aquatic Toxicology. 16SrRNA_(...) fasta and fastq formatted 16SrRNA sequences (Fig. 3c/ Table S4) with in the description line: * Colony numbers corresponding to the numbers included in Table S4 * The applied sequencing primer (27F) * NP exposure: nTiO2 (5 mg/L) or Control (no NPs) * A code referring to the parental zebrafish family that was crossed to obtain the larvae (A7, A8, or A9) 2photon_(...) * Two-photon scans of Fig. 2 * ROI sets of Fig. 2b/e (zipped) * Wavelength and focus information (.dat files) CLSM_(...) * CLSM scans of Fig. 3b (zipped) * ROI (zipped) * Metadata, where scanID corresponds to scan and ROI numbers of the zipped data CFUcounts_(...) CFU count data (Fig. 4/ Fig. S7): * Compound: Exposure: nTiO2 (5 mg/L), nAg (0.25 mg Ag/L), or control (no NPs) * LifeStage: Analyzed life stage: Embryo (1 dpf) or Larvae (5 dpf) * Date: Date of CFU isolation * DilutionFactor: Counted dilution * CFU_Larvae: CFU count per egg or larva DLS_(...) DLS measurements for nTiO2 (Table 2): * Time: Incubation time (h) * Concentration: Nominal concentration (mg/L) * ZP: Zeta potential (mV) * d_size: Z-averag size (nm) DoseResponses_(...) Mortality data of zebrafish larvae exposed to nTiO2 from 3-5 dpf (Fig. S6): * Concentration: Nominal exposure concentration (mg/L) * Day: Exposure time (days). * Mortality: Percentage of larvae that had died * Date: The date at which the mortality was scored EcoPlate_(...) EcoPlate measurement data (Fig. 5/ S3/ S8/ S9): * Compound: Zebrafish egg exposure: nTiO2 (5 mg/L), nAg (0.25 mg Ag/L), or Control (no NPs) * LifeStage: Analyzed life stage: Embryo (1 dpf) or Larvae (5 dpf) * Time: Incubation time (h) * Date: Date of the measurement * EcoPlate: Number of the EcoPlate * Measurement: Measurement number (3 measurements per plate) * 32 substrates (final 32 columns): OD at 590 nm measured for the given substrate PIXE_(...) PIXE measurements (Fig. 1/ Table S2): * ExposureConcentration: Nominal concentration of nTiO2 (mg/L) * Replicate: Replicate number * EggCount: Number of eggs that was analyzed * Date: Date at which the sample was taken * mL_EggWater: Volume of egg water (mL) that was analyzed along with the eggs * Element: Measured element * Total_ppm: Elemental concentration (ppm) * LOD_ppm: LOD (ppm) * ng_Element_per_egg: Elemental concentration (ng/egg) * LOD_ng_per_egg: LOD (ng/egg) TiO2Sedimentation_(...) ICP-OES measurements of Ti for sedimentation analyses (Fig. S4): * Time: Incubation time (h) * ExposureTiO2: Nominal concentration of nTiO2 (mg/L) * MediaTi: Measured concentration of Ti (mg/L) * MediaTiO2: Measured concentration of TiO2 (mg/L) UVspectra_(...) UV-light spectra (Fig. S1): * wavelength: Measured wavelength (nm) * irradiance: Measured irradiance (uW/cm2/nm) * spectrum: Number of the acquired spectrum
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