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Quantification of off-target events produced by site-directed RNA editing

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NIAID Data Ecosystem2026-03-10 收录
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https://www.ncbi.nlm.nih.gov/bioproject/PRJNA433471
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In these experiments we transfected HEK 293T cells with CFTR Y122X and various combinations of guide RNAs and site-directed RNA editing enzymes in order to correct the mutation. RNA was extracted from each condition and sequenced using the Illumina platform. DNA was also isolated and sequenced from a separate plate from the same cells.
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2018-02-08
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