Comparison of microRNA expression profiling in porcine brain among three time points after infection with Chinese I genotype strain of Toxoplasma gondii

The objectives of the present study were to analyze the changes on miRNA expression profile in porcine brain using next-generation sequencing (NGS) technology and bioinformatic analysis at 10, 25 and 50 days post inoculation (DPI) with Chinese I genotype strain of T. gondii. Compared to control group, 87, 68, and 135 differentially expressed miRNAs (DEMs) were found in the brains of infected pigs at 10, 25, and 50 DPI, respectively. The results not only showed that the miRNA expression of the host can be changed by the invasion of T. gondii, but also revealed the differences on the regulation of the key biological processes or pathways involved in host responses between the acute and chronic T. gondii infection. Overall design: Differential expression of porcine microRNAs at three time points after infection with Chinese I genotype strain of Toxoplasma gondii were analyzed by deep sequencing