T7 DNA polymerase in complex with a primer/template DNA containing a disordered N-2 aminofluorene on the template, crystallized with dideoxy-ATP as the incoming nucleotide.

T7 DNA polymerase in complex with a primer/template DNA containing a disordered N-2 aminofluorene on the template, crystallized with dideoxy-ATP as the incoming nucleotide. Descriptor: 5'-D(*CP*CP*CP*(AFG)*AP*TP*CP*AP*CP*AP*CP*TP*AP*CP*CP*AP*AP*TP*CP*AP*CP*TP*CP*TP*CP*C)-3', 5'-D(*GP*GP*AP*GP*AP*GP*TP*GP*AP*TP*T*GP*GP*T*AP*GP*TP*GP*TP*GP*AP*(2DT))-3', DNA polymerase, ... Authors: Dutta, S, Li, Y, Johnson, D, Dzantiev, L, Richardson, C.C, Romano, L.J, Ellenberger, T. Deposit date: 2004-08-24 Release date: 2004-10-26 Last modified: 2024-02-14 Method: X-RAY DIFFRACTION (2.3 Å) Cite: Crystal structures of 2-acetylaminofluorene and 2-aminofluorene in complex with T7 DNA polymerase reveal mechanisms of mutagenesis. Proc.Natl.Acad.Sci.Usa, 101, 2004

本数据集对应复合物为T7 DNA聚合酶（T7 DNA polymerase）与模板链携带无序N-2-氨基芴（N-2 aminofluorene）的引物/模板DNA复合物，该复合物以双脱氧ATP（dideoxy-ATP）作为待掺入核苷酸完成结晶。序列标注信息如下：5'-D(*CP*CP*CP*(AFG)*AP*TP*CP*AP*CP*AP*CP*TP*AP*CP*CP*AP*AP*TP*CP*AP*CP*TP*CP*TP*CP*C)-3'，5'-D(*GP*GP*AP*GP*AP*GP*TP*GP*AP*TP*T*GP*GP*T*AP*GP*TP*GP*TP*GP*AP*(2DT))-3'，样本组分包含DNA聚合酶（DNA polymerase）等。作者包括Dutta, S、Li, Y、Johnson, D、Dzantiev, L、Richardson, C.C、Romano, L.J、Ellenberger, T。提交日期：2004-08-24，发布日期：2004-10-26，最后修改日期：2024-02-14。实验方法为X射线衍射（X-RAY DIFFRACTION，分辨率2.3 Å）。引用文献：《与T7 DNA聚合酶结合的2-乙酰氨基芴与2-氨基芴的晶体结构揭示诱变机制》，发表于《美国国家科学院院刊（Proc. Natl. Acad. Sci. USA）》2004年第101卷。