RNA sequencing of suprachiasmic nucleus (SCN) of mice with rev-erb a/b knockout in GABAergic neuron

We compared differentially expressed genes (DEGs) in isolated SCN of female wild-type and GABAergic neuron rrev-erb a/b knockout (KO) mice at ZT12-14. 29 genes are significantly upregulated and 14 genes are statistically downregulated in SCN of KO mice. Aside from the circadian rhythm as an expected top-enriched functional entity, DEGs were enriched with cell surface proteins and associated modulators. Many of these genes are related to neurotransmission or neurological diseases. SCN were isolated at ZT12-14. Equal amount of RNA from 2 SCN were pooled for each sample. Three samples were analyzed for each genotype.本研究比较了在昼夜节律时间（Zeitgeber Time, ZT）12-14时段分离的雌性野生型与γ-氨基丁酸能神经元（GABAergic neuron）Rev-erb a/b敲除（knockout, KO）小鼠的视交叉上核（Suprachiasmatic Nucleus, SCN）内的差异表达基因（differentially expressed genes, DEGs）。KO小鼠的SCN中，共有29个基因显著上调，14个基因经统计学分析显示显著下调。除昼夜节律作为预期富集程度最高的功能类别外，差异表达基因还显著富集于细胞表面蛋白及其相关调控因子。其中多数基因与神经传递过程或神经系统疾病相关。本实验于ZT12-14时段分离SCN，每份样本混合取自2个SCN的等量总RNA，每种基因型均设置3个样本进行分析。