Nanopore signal and sequencing data for control and dA-AL-II-modified synthetic DNA generated using Oxford Nanopore and Qitan Technology platforms

This dataset supports the study of nanopore signal alterations and Z-score-based detection of dA-AL-II-modified synthetic DNA using Oxford Nanopore and Qitan Technology sequencing platforms. The dataset contains raw ionic-current signal files generated by both sequencing platforms, basecalled and reference-aligned sequencing reads, prepared reference-aligned signal matrices, a custom reference sequence, and processed position-specific sequencing-error and odds-ratio results. The Oxford Nanopore raw ionic-current data are provided as FAST5 files packaged in tar.gz archives. The corresponding basecalled and reference-aligned reads are provided separately as BAM files. The Qitan Technology raw ionic-current data are provided in the platform-specific H5 format. The corresponding basecalled and reference-aligned reads are provided separately as BAM files. The BAM files contain basecalled sequencing reads and per-base quality values aligned to the custom reference sequence provided as ref.fasta. The corresponding BAM index files are also included. The prepared signal matrices are provided as Python pickle files. Each pickle file contains a dictionary with a key named "sigs". The value associated with "sigs" is a two-dimensional reference-aligned signal matrix in which each row represents an individual read and six aligned signal measurements are used per reference position. The dataset includes two experimental conditions: an unmodified synthetic DNA control and a synthetic DNA construct containing a pre-synthesized dA-AL-II adduct incorporated into the DNA strand. The associated downstream analysis code is publicly available at: https://github.com/liuhaha0607/nanopore-zscore-analysis A versioned release of the analysis code is permanently archived in Zenodo: https://doi.org/10.5281/zenodo.21737768