Transcriptomic response to Keap1-specific electrophile modification in zebrafish embryos

Using our eletrophile delivery platform in zebrafish embryos (termed "Z-REX"), we launched a Z-REX-coupled RNA-seq screen to identify genes whose expression is significantly changed following Keap1-engagement with selective native lipid-derived electrophiles housing covalently-reactive motifs. The electrophiles tested were 4-hydroxynonenal (HNE) and 4-hydroxydodecenal (HDE). Overall design: Three samples were analyzed with one replicate per condition: non-treated fish, fish subjected to HNE Z-REX, and fish subjected to HDE Z-REX.