MAITs in HCC

Here we investigate the role of mucosal-associated invariant T (MAIT) cells in human and murine hepatocellular carcinoma (HCC). We isolated immune cells of syngeneic HCC cell line RIL-175 tumor-bearing or tumor-free livers and applied FACS sorting (CD45+ leukocytes) prior to 10X genomics based scRNA-seq. This is a complimentary dataset to the human scRNA-seq (deposited through dbGaP), highly multiplexed immunofluorescence microscopy using CODEX technology (deposited through TCIA) of paired patient samples. Experimental setup: 7 weeks old female C57BL/6 mice were injected intrahepatically with syngeneic HCC cell line RIL-175 or left tumor-free. At d28 mice were sacrificed and liver and tumor-infiltrating MNCs were isolated after in vivo digestion. CD45+ sorted cells were subsequently subjected to single-cell RNA-sequencing.