Richard Allen (2010) CIL:10004, Didinium nasutum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

An image of the pellicle of Didinium, in cross section of a non-dividing cell, has a plasma membrane with a distinct glycocalyx on its external surface and a flattened alveolar sac system. Under the alveoli are one or two or more ribbons of microtubules in ridges between the more corrugated mucocyst docking sites. Mitochondria with tubular cristae lie under the epiplasm in the ribosome filled cytosol. TEM taken on 2/18/69 by R. Allen with Philips 300 operating at 60kV. Neg. 20,500X. Bar = 0.5µm. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. A raw, unprocessed, high resolution version of this image (CIL:9927) is in the library and available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

一帧展示 Didinium 细胞非分裂阶段的细胞壁横切面图像，其中细胞质膜在其外部表面具有明显的糖萼结构，以及扁平的肺泡囊系统。在肺泡下方，存在一条或多条微管带，这些微管带位于更为波纹状的粘液囊肿对接位点之间的脊中。线粒体带有管状嵴，位于充满核糖体的胞浆的表质下方。此透射电子显微镜（TEM）图像由 R. Allen 于 1969 年 2 月 18 日使用 Philips 300 型电子显微镜在 60kV 下拍摄。负片放大倍数为 20,500 倍。标尺长度为 0.5 微米。负片经打印至纸张并扫描至 Photoshop 中。此数字化图像可用于定性分析。该图像的原始、未经处理的高分辨率版本（CIL:9927）存档于图书馆，可用于定量分析。采用标准戊二醛固定法，随后进行四氧化锇处理，用酒精脱水并浸渍于环氧树脂中。使用切片机制备的切片厚度约为 75 纳米。更多详细信息请参阅（http://www5.pbrc.hawaii.edu/allen/）。